Journal
/
Medicine
/
Quantifying Glomerular Permeability of Fluorescent Macromolecules Using 2-Photon Microscopy in Munich Wistar Rats
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Quantifying Glomerular Permeability of Fluorescent Macromolecules Using 2-Photon Microscopy in Munich Wistar Rats

Quantifying Glomerular Permeability of Fluorescent Macromolecules Using 2-Photon Microscopy in Munich Wistar Rats

DOI:
10.3791/50052-v

•

11:13 min

•

April 17, 2013

•

,
1Medicine/Nephrology,Indiana University School of Medicine

Chapters

  • 00:05Title
  • 01:50Exposing the Kidney in a Munich Wistar Rat for Intravital 2-Photon Imaging
  • 04:10Acquiring Images to Quantify Renal Permeability of Albumin
  • 06:02Calculating Glomerular Sieving Coefficient for Fluorescent Albumin from 3D Volumes
  • 08:21Glomerular Permeability Results
  • 10:34Conclusion

Summary

Automatic Translation

Automatic Translation

A technique utilizing high resolution intavital 2-photon microscopy to directly visualize and quantify gloemrular filtration in surface glomeruli. This method allows for direct determination of permeability characteristics of macromolecules in both normal and diseased states.

Tags

Keywords: 2-photon MicroscopyGlomerular PermeabilityFluorescent MacromoleculesMunich Wistar RatsKidney DiseasesUrinary LossSerum AlbuminPodocytesVascular Endothelial CellsBasement MembraneGlomerular Filtration BarrierProximal Tubule CellsMicropunctureDextran PolymersBowman’s SpaceAlbumin ReabsorptionTubular TranscytosisProteinuric Renal Diseases

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/50052?article_type=v

Leprdb Mouse Model of Type 2 Diabetes: Pancreatic Islet Isolation and Live-cell 2-Photon Imaging Of Intact Islets
check_url/50052?article_type=v

Intravital Microscopy of Tumor-associated Vasculature Using Advanced Dorsal Skinfold Window Chambers on Transgenic Fluorescent Mice
check_url/50052?article_type=v

A Method for Quantifying Upper Limb Performance in Daily Life Using Accelerometers
check_url/50052?article_type=v

In Vivo Morphometric Analysis of Human Cranial Nerves Using Magnetic Resonance Imaging in Menière’s Disease Ears and Normal Hearing Ears
check_url/50052?article_type=v

Transdermal Measurement of Glomerular Filtration Rate in Mice
check_url/50052?article_type=v

Highly Sensitive Measurement of Glomerular Permeability in Mice with Fluorescein Isothiocyanate-polysucrose 70
check_url/50052?article_type=v

Whole-Mount Immunofluorescence Staining, Confocal Imaging and 3D Reconstruction of the Sinoatrial and Atrioventricular Node in the Mouse
check_url/50052?article_type=v

Isolation and Culture of Resident Cardiac Macrophages from the Murine Sinoatrial and Atrioventricular Node
check_url/50052?article_type=v

Analyzing Long-Term Electrocardiography Recordings to Detect Arrhythmias in Mice
check_url/50052?article_type=v

Using 2-Photon Microscopy to Quantify the Effects of Chronic Unilateral Ureteral Obstruction on Glomerular Processes

Read Article